DNA samples are loaded to 96-well plate for PCR analysis

Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.

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iRepertoire poster entitled iR-HLA cassette: An automated and integrated solution for NGS based HLA-typing

Dig into our knowledge base through exploring our immune research posters. Here you can view and download our recently presented posters.

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graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.

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pcr strip and biological microscope

Immune repertoire sequencing: RepSeq and RepSeq+ iRepertoire’s sequencing services RepSeq and RepSeq+ complement our amplification technologies arm-PCR and dam-PCR, respectively. Because arm-PCR and dam-PCR have different strengths, the decision of whether to use RepSeq or RepSeq+ will depend on the nature of your specific project.  Arm-PCR uses internal and external nested primers for extremely inclusive…

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graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

Why single cells? Information related to the physical, cognate pairing of TCR-alpha and -beta chains or Ig-heavy and -light chains is lost once RNA extraction is performed on a bulk cell sample. iPair, our single cell immune sequencing service, was developed so that the repertoire of individual cells could be profiled through capture of chain…

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User interface of the iPair Analyzer bioinformatics demo

All of our services come with complimentary basic data analysis through our proprietary pipeline, which then outputs data to iRweb. iRweb is a web-based RepSeq bioinformatic software that allows researchers to have all of the following services and analyses performed on their samples: Barcode demultiplexing The number of CDR3s captured in the library and the…

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Experienced businesswoman reading publication via laptop computer making research for planning startup at desktop,female secretary checking mail on netbook organizing working process in office

Overview This page describes the technical steps involved in iRweb’s bioinformatic pipeline. If you are unfamiliar with data analysis for next-generation sequencing, we suggest you visit our NGS overview page. For a general introduction to iRweb, see our Learning Center article about data analysis. For more details about iRweb than what can be found on…

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