Efficiently discover rare T and B cell clones for immunotherapy development with iPair
Discover full-length V(D)J sequences from rare T and B cells at single-cell resolution
T cell receptor (TCR) and B cell receptor (BCR) therapeutics are opening new opportunities to treat serious human health issues such as infectious and autoimmune diseases and cancer. However, finding the right TCR and antibody is challenging, especially if it is present in or produced by rare T and B cell populations.
Single-cell sequencing has provided key data about TCR and BCR sequences, but current workflows involve multiple, separate reactions in large reaction volumes, significantly driving up costs and time. At iRepertoire, we created iPair, the first commercialized technology for capturing physically paired full-length V(D)J sequences in TCRs or BCRs from FACS isolated single cells in the same reaction well. iPair uses a low reaction volume, enabling cost-effective, paired-chain TCR and BCR sequencing at the single-cell level.
Reliably capture physically paired TCR and BCR chains variable regions
Powered by our ultrasensitive arm-PCR technology, iPair enables targeted amplification of immune receptor heterodimers from a single cell. See the data below to explore how our proprietary method ensures the sensitive and accurate sequencing of rare T and B cell subsets.
As a proof-of-concept experiment, Jurkat cells were stored in QIAgen’s RNA Protect buffer for one week at 4ºC, then plated into an iCapture 96-well plate. To replicate less-than-ideal shipping conditions, the plate was kept in a car for two days in the beautiful–but notoriously hot–Alabama summer. The plate was then processed through our iPair service where we were able to amplify the TCRB for >90% of the samples.
Targeted single-cell sequencing that seamlessly integrates into existing FACS workflows
Because of its simplicity, iPair can easily integrate into any fluorescence-activated cell sorting (FACS) workflow. By utilizing dry capture and miniaturized reaction volumes, iPair overcomes the major pitfalls of wet capture methods, such as diluted signals, increased reagent consumption, and multiple plate usage.
iPair’s targeted approach ensures high pairing efficiency at a low operating cost by minimizing sample loss, which is highly prevalent in microfluidics encapsulation and adapter ligation workflows.
Optimized T and B cell lead discovery workflow for accurate identification of therapeutic candidates
- Accurate rare T cell and B cell receptor sequencing
- Cost-efficient lead discovery
- Optimized workflow that delivers candidate TCR and BCR fast
Empowering the future of medicine one repertoire at a time
TCR gene therapy
Identify tumor-specific TCRs for CAR and TCR therapy
Antibody-based therapeutics
Capture antigen-specific rare B-cells for monoclonoal antibody therapy development
Flexible solutions that adapt to all needs
Getting started is easy. There are three ways you can share samples with us:
Sort cells into 96-well iCapture plates:
Single sort at your site using the iCapture kit and send the plate to us for service. A no-charge validation of your sorter and method with our process is included.
Sort cells in a tube:
Sort a subpopulation of your cells into Quiagen’s RNAprotect cell reagent and ship the tube to us at 4 °C. We will single-cell plate cells at iRepertoire’s service laboratory.
No sorting:
If you lack sorting capabilities, we can handle cryopreserved bulk populations of cells and single-sort the ones that interest you. For this modality, a project consultation is needed.
Expand your single cell analysis with iPair + Immunosight
Expand the reach of your single-cell sequencing analysis with Immunosight, our customizable targeted gene amplification panel. With iPair + immunosight, you will receive physically paired TCR and BCR chain sequence insights with gene expression data of 150-plus genes carefully selected to cover diverse cell functions and markers. You can customize our panel according to your research needs.
iPair in action
Predicting and measuring patient response to cell therapy with single cell TCR sequencing
Using iPair-TCR™ and iPair-TCR+™ to track tumor infiltrating lymphocytes from peripheral blood in a longitudinal breast cancer patient study (2017)
Miranda L. Byrne-Steele et al., Presented: SITC 2017