Discover Your Next Immunotherapy Biomarker with Quantitative Adaptive Immune Repertoire Profiling

Biomarker discovery from adaptive immune receptor repertoire (AIRR) data is both technically and analytically demanding:

PCR amplification artifacts and sequencing noise can obscure true biological signals.
Highly diverse repertoires introduce sampling bias and reproducibility challenges.
Extracting interpretable patterns from large, complex AIRR datasets requires bioinformatics capabilities that most teams don’t have in-house.

Our platform combines proprietary multiplex PCR chemistry, comprehensive multi-chain sequencing, and purpose-built bioinformatics workflows to deliver precise, reproducible insights into adaptive immune dynamics across patients, timepoints, and sample types. Whether you are investigating clonal expansion in early-phase trials, characterizing immune remodeling in resistance-associated tumor profiles, or identifying meaningful immune signatures linked to efficacy, toxicity, and disease progression, iRepertoire provides the clarity and confidence your program requires.

Track T-Cell Repertoire Dynamics to Reveal Immunotherapy Response Biomarkers

Detecting TCR repertoire remodeling in patients who don't respond to standard immunotherapy

As immuno-oncology enters a precision era, the demand for biomarkers that explain response, resistance, and durability has grown rapidly. Yet commonly used markers such as PD-L1 expression or tumor mutational burden often fail to fully predict clinical outcomes. See how our platform enabled product characterization, clonal tracking, and mechanistic biomarker generation in patients with metastatic non-small cell lung cancer.

Key findings:

Post-infusion peripheral blood clonality more closely resembled the TIL product than pre-infusion blood, confirming TCR repertoire remodeling driven by the therapy
TIL product clone expansion was detected in post-infusion blood across patient subgroups, including those with resistance-associated tumor profiles
iRepertoire’s technology enables precision medicine through highly accurate TCR/BCR sequencing and comprehensive, quantitative data analysis tailored for biomarker detection

See how precise, quantitative AIRR analysis turns clonal dynamics and repertoire remodeling into clinically actionable biomarkers

CRISPR-Cas9 CISH-knockout TIL therapy achieves complete response in immunotherapy-resistant metastatic colorectal cancer

Targeting the intracellular immune checkpoint CISH with CRISPR-Cas9-edited TILs in patients with metastatic colorectal cancer.

iRepertoire TCR profiling confirmed TIL polyclonality and neoantigen specificity in the first-in-human phase 1 trial of CISH-knockout cell therapy.

TCR repertoire profiling characterizes clonal dynamics and persistence in autologous TIL therapy for mNSCLC

Lifileucel, an autologous TIL monotherapy, was evaluated in patients with advanced NSCLC resistant to immune checkpoint inhibitors. RepSeq enabled product QC and quantitative TCR clonotype tracking, supporting mechanistic biomarker development in a Phase 2 clinical trial.

Seven-chain adaptive immune receptor repertoire analysis in rheumatoid arthritis reveals novel features associated with disease and clinically relevant phenotypes

Comprehensive 7-chain AIRR analysis of samples obtained from RA patients revealed disease-associated clonal signatures and diversity indices as potential biomarkers across a large patient cohort. Data showed clear differences between therapeutic responders and non-responders.

Immune repertoire profiling reveals TCR signatures associated with clinical outcomes following immune checkpoint inhibitor treatment

Immune repertoire profiling of pre- and post-treatment samples identified novel TCR signatures associated with positive clinical outcomes in ICI-treated (immune checkpoint inhibitor) patients.

Explore our services

Bulk immune repertoire profiling

Discover RepSeq+, our sensitive, quantitative, and high-throughput assay that combines multiplex PCR with NGS to analyze any combination of all seven chains of the adaptive immune system in a single reaction.

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Single cell V(D)J profiling

Explore iPair, the first commercialized technology for capturing physically paired full-length V(D)J sequences in TCRs or BCRs from FACS isolated single cells in the same reaction well.

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Data Analysis

Leverage our built-for-purpose, advanced AIRR analysis tools that translate raw sequence data into actionable insights.

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Additional resources

Ready to get started?

iRepertoire serves as a leader in the field of immune repertoire profiling. We invite you to consult with our team of scientists to discover how our services can accelerate your analysis of immune cell function and diversity.