Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™
Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™
Rare, self-reactive B and T cell clones drive autoimmune diseases like Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), Celiac Disease (CD), and Type 1 Diabetes (T1D), but often elude conventional adaptive immune repertoire profiling. Identifying these low-frequency clonotypes, along with their isotype usage and mutation patterns, is essential for advancing biomarker discovery and therapeutic development.
Our highly sensitive adaptive immune receptor repertoire (AIRR) profiling services deliver the deep coverage and isotype-level resolution needed to uncover clonal-level insights. With seven-chain profiling, single-cell paired chain resolution, and expert analytical support, we help autoimmune drug development teams discover biomarkers, understand mechanisms of action, and develop more precise, targeted therapies.
Autoimmune diseases are the third most common cause of chronic illness in the US, yet their mechanisms remain incompletely understood. The clonotypes that drive pathology are often rare, transient, and below the detection threshold of standard cell-based assays — making them easy to miss and difficult to interpret without the right tools.
Autoimmune diseases are complex, multi-factorial, and dynamic. With immune profiling, it is possible to precisely monitor changes in immune cell diversity over time and post-therapy to identify biomarkers, inform clinical study design, and predict therapeutic response.
This article identifies and characterizes two distinct subpopulations of marginal zone (MZ) B cells in mice—CD80^high^ and CD80^low^—which differ in antibody-producing capacity, radioresistance, and autoreactivity, illuminating new dimensions of B cell biology and adaptive immunity.
iRepertoire’s customizable solutions were especially critical for deep molecular comparison from rare or complex sample types, supporting translational studies on immune differentiation and tolerance.
This article presents a multi-omics study that uncovers pathogenesis differences in skin lesion generation between systemic lupus erythematosus (SLE) and idiopathic discoid lupus erythematosus (IDLE), using immune repertoire sequencing to show distinct B cell and T cell clonal landscapes that support precision diagnosis and therapeutic approaches.
Immune repertoire profiling was critical for distinguishing clonal architecture and immune diversity in SLE versus IDLE, establishing direct relationships between molecular findings and pathogenetic mechanisms.
This article demonstrates that molecular mimicry-driven autoimmunity plays an important role in chronic rhinosinusitis with nasal polyps (CRSwNP), using immunoglobulin repertoire sequencing to reveal dominant B cell clones targeting both self and microbial antigens in affected tissue.
The use of iRepertoire’s workflows supported efficient antigen discovery and detailed characterization of the humoral immune repertoire, setting the stage for translational investigation and biomarker development in airway autoimmunity.
This article introduces immuneREF, a novel computational framework for multidimensional, reference-based comparison of adaptive immune receptor repertoires. The study reveals that blood-derived immune repertoires from healthy and diseased populations are much more similar than previously assumed—highlighting only subtle differences across autoimmune conditions and infections.
Immune repertoire analysis is pivotal for understanding the molecular and clonal landscape of B and T cell diversity, enabling discovery of subtle immune changes linked to health or disease.
Discover RepSeq+, our sensitive, quantitative, and high-throughput assay that combines multiplex PCR with NGS to analyze any combination of all seven chains of the adaptive immune system in a single reaction.
Explore iPair, the first commercialized technology for capturing physically paired full-length V(D)J sequences in TCRs or BCRs from FACS isolated single cells in the same reaction well.
Leverage our built-for-purpose, advanced AIRR analysis tools that translate raw sequence data into actionable insights.
iRepertoire serves as a leader in the field of immune repertoire profiling. We invite you to consult with our team of scientists to discover how our services can accelerate your analysis of immune cell function and diversity.