Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™

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iRepertoire, Inc.

Hybridoma Sequencing Capabilities

Secure, high-quality antibody sequencing from the immune sequencing experts

Hybridoma cell lines are one of the most efficient sources of antibody production, but they can be difficult and expensive to establish and maintain. By sequencing your Hybridoma cell lines you can:

  • Increase antibody security. If the cell line is lost, the antibody can be made recombinantly from the sequence.
  • Characterize the antibodies prior to downstream functional assessment to save money (i.e.duplicate clones identified and removed early).
  • Decrease loss risk by digitizing your antibody library for safe keeping.
  • Explore opportunities for antibody sequence modifications such as humanization or isotype changes.
  • Streamline IP and patent filings.
  • Validate your antibody sequences.
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Hybridoma Sequencing Service

Optimize your production process, reduce your risk, and secure your intellectual property

  • Easy sample submission: simply send RNA from the hybridoma line or frozen cells
  • Affordable and scalable sequencing
  • View our brochure for more information on iRep’s hybridoma sequencing service
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Hybridoma Sequencing Kit

Leverage our MonoVDJseq optimized BCR library preparation kit for high throughput antibody sequencing

  • The MonoVDJseq reagent kit provides optimized reagents, target enrichment primers for BCR 3-chain (H + K + λ), and Illumina® sequencing adapters for in- house library preparation.
  • Proprietary 3-in-1 IgH/k/λ amplification combined with distinct dual index ranges allows multiplexing of multiple 96-well plates within a single sequencing flow cell.
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Why iRepertoire?

  • 15+ years of immune seq experience
  • Affordable sequencing
  • Easily scalable for large libraries
  • Over 8,000 hydridomas sequenced
  • High-throughput using NGS
  • NGS paired chained analysis

Connect with an immune sequencing expert today.

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