Technology

Inspiring clinically-actionable discoveries in immune biology

Sequence the full variable region of all 7 chains for single cells or bulk repertoires

An immune repertoire is the sum of functionally diverse T and B cells in a body at any given moment. Sampling
the immune repertoire provides both a snapshot and a historical record of immune function, enabling an advanced understanding of disease and treatment response.

Over the last 10 years, iRepertoire has developed the gold standard technologies for immune repertoire sequencing and continues to innovate new solutions for profiling the adaptome. Our core technologies provide the ability to amplify all of the expressed V(D)Js in B or T cells, and we offer the only technology that can measure all 7 immune chains in a single reaction. iRepertoire is committed to the creation of cutting-edge technology that adapts to the
ever-changing immune sequencing landscape.

arm-PCR

Amplicon Rescue Multiplex PCR

arm-PCR inclusively amplifies all of the expressed V(D)Js in B or T cells for cost-efficient insights into the expressed immune repertoire. arm-PCR is ideal for capturing rare cell types, tetramer analysis, and single cell applications even for low volume and difficult to handle sample types.

dam-PCR

Dimer avoided multiplex PCR

dam-PCR enables highly quantitative amplification of any combination of TCR and BCR chains (alpha, beta, delta, gamma, IgH, and kappa/lambda) in a single reaction. dam-PCR is ideal for isotype and clonotype frequency analysis, hyper-mutation status, and FFPE-damaged samples.

Streamlined single cell immune sequencing for paired chain and phenotypic analysis

Single cell immune sequencing preserves information about the cognate pairing of BCR and TCR chains. Paired TCR and BCR chains are shown in the wells of a stylized iPair plate.
V(D)J RNA amplified and sequenced

Our iPair single cell immune sequencing technology is the first commercially available service specifically designed for capturing physically paired variable regions in T cells and B cells. Our iPair+ single cell immune phenotyping technology allows for co-expression analysis of over 100 phenotypic genes.

iPair Applications

  • Paired chain analysis
  • CAR-T development
  • Lymphocyte tracking
  • Antibody reverse engineering
  • Rare cell analysis

Enabling flexible inputs, more insights, and efficiency

iRepertoire immune sequencing | Competitor analysis

Coverage
iRepertoire
Technology arm-PCR and dam-PCR
BULK SEQUENCING AVAILABLE? Yes
SINGLE CELL SEQUENCING AVAILABLE? Yes
HUMAN CDR CDR1, CDR2, & CDR3
MOUSE CDR CDR1, CDR2
IGH Human & Mouse
IGL Human & Mouse
IGK Human & Mouse
TCRB Human & Mouse
TCRA Human & Mouse
TCRD Human & Mouse
TCRG Human & Mouse
BCR ISOTYPE Yes
UMI AVAILABLE? Yes
INPUT (RNA/DNA) RNA or DNA
PRICE $
Competitor A
Technology multiplex-PCR
BULK SEQUENCING AVAILABLE? Yes
SINGLE CELL SEQUENCING AVAILABLE? No
HUMAN CDR CDR3 only
MOUSE CDR CDR3 only
IGH Human & Mouse
IGL Not Covered
IGK Not Covered
TCRB Human & Mouse
TCRA Human
TCRD Human
TCRG Human
BCR ISOTYPE No
UMI AVAILABLE? No
INPUT (RNA/DNA) DNA
PRICE $$
Competitor B
Technology template switch
BULK SEQUENCING AVAILABLE? Yes (10K cell max)
SINGLE CELL SEQUENCING AVAILABLE? Yes
HUMAN CDR CDR1, CDR2, & CDR3
MOUSE CDR CDR1, CDR2, & CDR3
IGH Human & Mouse
IGL Human & Mouse
IGK Human & Mouse
TCRB Human & Mouse
TCRA Human & Mouse
TCRD Human & Mouse
TCRG Human & Mouse
BCR ISOTYPE Yes
UMI AVAILABLE? Yes
INPUT (RNA/DNA) RNA
PRICE $$$

Ready to get started?