Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™
Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™
Adaptive immune receptor repertoire (AIRR) sequencing generates rich and expansive datasets, but capturing actionable biological and clinical insights requires extensive computational expertise. Common analysis pipelines often stop at sequence mapping and rudimentary diversity metrics, leaving critical information such as rare clonotypes, clonal evolution, and antigen-driven selection hidden.
Intellimune™ is designed to bridge the gap between immune repertoire data and repertoire intelligence. Built by immunology and computational experts, our service combines proven bioinformatics workflows with advanced visualization and expert interpretation to transform standard sequencing data into clear, reproducible, and decision-ready insights. Whether you are exploring immune diversity, tracking clonal dynamics in clinical studies, or identifying biomarkers of response and toxicity, Intellimune enables you to:
From single-cell clonal discovery to patient immune repertoire response, Intellimune transforms AIRR sequencing data into clear insights that accelerate biomarker discovery, therapeutic development, and clinical decision-making.
Included in all studies is Intellimune Standard, our reproducible data analysis workflow that provides the following easy-to-interpret outputs:
Intellimune Standard provides foundational repertoire characterization across TCR and BCR datasets, and it is best for first-time users conducting initial exploratory studies. Keep browsing to explore Intellimune Plus and Pro workflows for comprehensive data analysis and insight generation.
Intellimune scales with your needs helping you move faster from data to discovery. Click on the cards below to see how Intellimune Plus or Pro provide comprehensive repertoire insight generation, fast.
Generate high-confidence, insights with deeper characterization of immune diversity and clonality.
Uncover deep biological signals — including immune evolution, therapeutic response, and biomarker candidates — across patients and timepoints.
For translational research questions and clinical applications, Intellimune Discovery provides fully customized, expert-guided analysis tailored to your study goals. From identifying response and resistance mechanisms to tracking pathogenic clones, we collaborate with you to design, analyze, and interpret AIRR datasets in a translational and clinically relevant context. Common applications for Intellimune Discovery include:
Accelerate decision-making with tailored insights aligned to your translational and clinical development objectives. Click below to learn more about our Intellimune Discovery service.
Intellimune provides a comprehensive suite of metrics and visualizations designed to capture immune diversity, clonality, and functional dynamics. See below examples of key outputs across analysis services.
Treemaps shows the frequency and distribution of unique clonotypes. Each shape represents a clone, with size proportional to its abundance enabling rapid identification of clonal expansion.
With multi-chain treemaps you can visualize all seven receptor chains simultaneously, revealing relative expression and immune architecture across the full adaptome.
Single-chain treemap
Multi-chain treemap
This report shows the expanding and contracting of clones found for TRB for one individual after COVID vaccination using edgeR, with adjusted p-values and fold change for each clone.
Report that shows isotype (C-gene) usage of IgM, IgD, IgG, IgE, IgA, and isotype-related metrics, e.g., Class switch recombination (CSR) index and percentage of mutated and unmutated naïve IgD/IgM sequences will be derived from isotype mapping data. The Somatic hypermutations (SHM) score for each unique sequence in a repertoire will be calculated by measuring the percentage of mismatches (mutations) between a data sequence and its V reference. The mean of all sequences in a sample represents a repertoire’s SHM rate.
This report provides a wide range of diversity analysis and metrics to capture the distribution of clonotypes of a sample and global changes in immune response, such as:
These various metrics increase confidence that the diversity is being appropriately measured, and differences may reflect nuances concerning the repertoire that would be masked otherwise.
New computational models allow for Ag-specific prediction of individual TCRs within repertoire datasets, particularly when training data specific to peptides is generated for a subset of patient samples.
During exploratory analysis, observe differences between groups of samples by comparing the statistical relationship of pre-calculated data points to answer hypothesis-driven questions.
Intellimune transforms complex AIRR datasets into intuitive visualizations that make immune dynamics immediately clear. From clonal expansion patterns to isotype switching and lineage evolution, these outputs help you identify meaningful biological signals that drive discovery and clinical progress.
Adapted from Atriedo et. al, various diversity indices were used alongside bubble plots to depict the repertoires of RA patients versus healthy controls and post TNFα inhibition therapy.
Adapted from Li et. al, SMH%s and CSR were used to distinguish PBMC and skin lesions BCR repertoires in patients with Systemic Lupus Erythematosus (SLE) and Isolate discoid LE (IDLE). Furthermore, network plots of the related clonotypes colored by isotype were compared in the skin lesions of both IDL and SLE.
Adapted from Li et al., and Atreido et al., On the left a multi-chain tree plot is shown. Each quadrant corresponds to a specific adaptive immune receptor chain as shown on the diagram. Each shape represents a clone, with size proportional to its abundance. On the right, specific clones can be tracked across dilutions.
iRepertoire’s Quality Management System adheres to GCLP standards, ensuring its innovative products and services are of the highest quality and meet or exceed customer requirements and expectations.
Our commitment includes:
Our commitment to Continual Quality Improvement (CQI) includes continuous staff training, state-of-the-art equipment, and rigidly maintained systems. These measures effectively and efficiently control product supply and project completion in a timely, accurate, coherent, relevant, and professional manner.