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Learn more about immune biology, repertoire sequencing, and iRepertoire’s solutions for characterizing immune diversity.

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Immunology basics
T-and-B-cell-graphic-01

T cell and B cell Overview

BCR-germline-V8

Diversity and differentiation in the adaptive immune system

CDR3-distribution-graphic-1

The immune repertoire and adaptome

NGS basics
Sequencing-Workflow-1

NGS overview: from sample to sequencer to results

NGS sequencing coverage process

NGS considerations: coverage, read length, multiplexing

iRepertoire: Immunology meets NGS
Tcells_attacking_cancer_cell

Why sequence the immune repertoire?

clonotype distribution in one person. Long-tailed negative distribution

How to sequence the immune repertoire

primer-graphics

iRepertoire’s primer systems

FirstRoundPCR_V2

iRepertoire’s amplification technologies

pcr strip and biological microscope

iRepertoire’s sequencing services

graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

iPair: iRepertoire’s single cell service

iPair Analyzer monitor view.

iRepertoire’s data analysis services

Experienced businesswoman reading publication via laptop computer making research for planning startup at desktop,female secretary checking mail on netbook organizing working process in office

iRweb: Technical notes

Project planning, sample preparation and submission
pcr strip and biological microscope

Choice of starting template: RNA vs gDNA vs cDNA

MiSeq_2

Choosing your read depth

DNA samples are loaded to 96-well plate for PCR analysis

Pooling samples

graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

Cell-sorting guide

sample-prep-graphics-01-2

Blood collection and sample considerations

rosebox-BFdSCxmqvYc-unsplash

Shipping samples, receiving products, and submitting data

Pipette_tube_in_hood

Sample submission guide – bulk repertoire

Publications, posters, and patents
iRepertoire poster entitled iR-HLA cassette: An automated and integrated solution for NGS based HLA-typing

Posters

Experienced businesswoman reading publication via laptop computer making research for planning startup at desktop,female secretary checking mail on netbook organizing working process in office

Publications and patents