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Learn more about immune biology, repertoire sequencing, and iRepertoire’s solutions for characterizing immune diversity.

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Immunology basics

Structure of T cell and B cell receptors. T cell receptors are made up of two polypeptide chains that together compose one antigen binding region. B cell receptors are made up of four peptides – two light chains and two heavy chains – that comprise two antigen-binding regions.

B cell and T cell structure and function

Diversity and differentiation in the adaptive immune system

Clonotype: A particular CDR3 sequence. Repertoire: All of the clonotypes within a particular BCR or TCR chain. Adaptome: All of the repertoires for each of the 7 different chains.

The immune repertoire and adaptome

NGS basics

Next-generation sequencing workflow: 1) Extraction 2) Library Prep 3) Sequencing 4) Analysis

NGS overview: from sample to sequencer to results

Simple graphic describing the difference between high and low coverage regions in next generation sequencing: higher coverage equates to more reads

NGS considerations: coverage, read length, multiplexing

Immunology meets NGS: iRepertoire's Technology

MiSeq machines

Beyond template switching

T cells attacking cancer cell, why sequence the immune repertoire

Why sequence the immune repertoire?

Clonotype distribution in one person. Long-tailed negative distribution with number of clonotypes on the y-axis and nuber of aliquots on the x-axis. When immune repertoire sequencing, you want to cover this highly variable CDR3 region.

How to perform immune repertoire sequencing

Primer Systems

iRepertoire’s primer systems

In the first round of arm-PCR, inside and outside TCR/BCR-specific primers amplify the targets of interest, increasing sensitivity and appending communal primer binding sites

iRepertoire’s amplification technologies

Immunology meets NGS: iRepertoire's Services

pcr strip and biological microscope

iRepertoire’s immune sequencing services

graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

iPair: iRepertoire’s single cell immune sequencing service

User interface of the iPair Analyzer bioinformatics demo

iRepertoire’s data analysis services

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iRweb: Technical notes

Project planning, sample preparation and submission

pcr strip and biological microscope

Choice of starting template: RNA vs gDNA vs cDNA

MiSeq machines

Choosing your read depth

DNA samples are loaded to 96-well plate for PCR analysis

Pooling samples

graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

Cell-sorting Guide

Blood Collection Breakdown

Blood collection and sample considerations

Biotech sample brown cardboard shipping package

Shipping samples, receiving products, and submitting data

Pipette_tube_in_hood

Sample submission guide – bulk repertoire

Publications and posters

iRepertoire poster entitled iR-HLA cassette: An automated and integrated solution for NGS based HLA-typing

Posters

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Publications

White papers, case studies, and eBooks

iRepertoire eBook 3: Planning your immune repertoire sequencing experiment

eBook 3: Planning your immune repertoire sequencing experiment

iRepertoire's eBook 2: Intro to immune sequencing with iRepertoire thumbnail

eBook 2: Immune sequencing with iRepertoire

Case Study thumbnail: The evolution of antibody-based treatments for infectious disease, from convalescent plasma to single cell immune sequencing

Case Study: The evolution of antibody-based treatments for infectious disease

iRepertoire eBook 1: An Intro to Immunology and Immune Sequencing cover thumbnailiRepertoire eBook 1: An Intro to Immunology and Immune Sequencing. Fill out the form to download.

eBook 1: An Intro to Immunology and Immune Sequencing

Thumbnail of the first page of the Cell Therapy Case Study: Predicting and measuring patient response to cell therapy with single cell TCR sequencing

Case Study: Predicting and measuring patient response to cell therapy with single cell TCR sequencing

SITC Case Study: Immune Repertoire Sequencing for Pre- and Post-Treatment Monitoring in Cancer thumbnail. Download the full case study now.

Case Study: Immune Repertoire Sequencing for Pre- and Post-Treatment Monitoring in Cancer

Product manuals

Guides

Data Sheets