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Learn more about immune biology, repertoire sequencing, and iRepertoire’s solutions for characterizing immune diversity.

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Immunology basics
Structure of T cell and B cell receptors. T cell receptors are made up of two polypeptide chains that together compose one antigen binding region. B cell receptors are made up of four peptides – two light chains and two heavy chains – that comprise two antigen-binding regions.

T cell and B cell Overview

Germline configuration of antibody gene including V(D)J segments and constant regions with zoom in on the variable region and CDR3 and diagram of fully-spliced VDJ RNA

Diversity and differentiation in the adaptive immune system

Clonotype: A particular CDR3 sequence. Repertoire: All of the clonotypes within a particular BCR or TCR chain. Adaptome: All of the repertoires for each of the 7 different chains.

The immune repertoire and adaptome

NGS basics
Next-generation sequencing workflow: 1) Extraction 2) Library Prep 3) Sequencing 4) Analysis

NGS overview: from sample to sequencer to results

Simple graphic describing the difference between high and low coverage regions in next generation sequencing: higher coverage equates to more reads

NGS considerations: coverage, read length, multiplexing

Immunology meets NGS: iRepertoire's Technology
MiSeq machines

Beyond template switching

T cells attacking cancer cell, why sequence the immune repertoire

Why sequence the immune repertoire?

Clonotype distribution in one person. Long-tailed negative distribution with number of clonotypes on the y-axis and nuber of aliquots on the x-axis. When immune repertoire sequencing, you want to cover this highly variable CDR3 region.

How to perform immune repertoire sequencing

All of iRepertoire’s primer systems for immune repertoire sequencing cover the highly variable CDR3 region

iRepertoire’s primer systems

In the first round of arm-PCR, inside and outside TCR/BCR-specific primers amplify the targets of interest, increasing sensitivity and appending communal primer binding sites

iRepertoire’s amplification technologies

Immunology meets NGS: iRepertoire's Services
pcr strip and biological microscope

iRepertoire’s immune sequencing services

graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

iPair: iRepertoire’s single cell immune sequencing service

User interface of the iPair Analyzer bioinformatics demo

iRepertoire’s data analysis services

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iRweb: Technical notes

Project planning, sample preparation and submission
pcr strip and biological microscope

Choice of starting template: RNA vs gDNA vs cDNA

MiSeq machines

Choosing your read depth

DNA samples are loaded to 96-well plate for PCR analysis

Pooling samples

graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

Cell-sorting Guide

The makeup of "whole blood" infographic. Whole blood contains red blood cells (RBCs), white blood cells (WBCs), platelets, and plasma. Plasma contains protein and additional nutrients. The WBCs include both peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells (PMNCs). Lymphocytes (T cells, B cells, NK cells), monocytes, and macrophages make up the PBMCs, while neutrophils, eosinophils, basophils, and mast cells make up PMNCs.

Blood collection and sample considerations

Biotech sample brown cardboard shipping package

Shipping samples, receiving products, and submitting data

Pipette_tube_in_hood

Sample submission guide – bulk repertoire

Publications, posters, and patents
iRepertoire poster entitled iR-HLA cassette: An automated and integrated solution for NGS based HLA-typing

Posters

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Publications and patents

White papers, case studies, and eBooks
iRepertoire eBook 1: An Intro to Immunology and Immune Sequencing cover thumbnailiRepertoire eBook 1: An Intro to Immunology and Immune Sequencing. Fill out the form to download.

eBook 1: An Intro to Immunology and Immune Sequencing

Thumbnail of the first page of the Cell Therapy Case Study: Predicting and measuring patient response to cell therapy with single cell TCR sequencing

Case Study: Predicting and measuring patient response to cell therapy with single cell TCR sequencing

SITC Case Study: Immune Repertoire Sequencing for Pre- and Post-Treatment Monitoring in Cancer thumbnail. Download the full case study now.

Case Study: Immune Repertoire Sequencing for Pre- and Post-Treatment Monitoring in Cancer