Case study: Mitigating AIRRseq Challenges in FFPE Tissue Samples with the RepSeq+ Immune Repertoire Intelligence Platform​

Obtaining reliable seven-chain AIRRseq from challenging FFPE samples

Formalin-fixed paraffin-embedded (FFPE) tissues are valuable for immune profiling but pose significant technical challenges for adaptive immune receptor repertoire sequencing (AIRRseq), including RNA fragmentation, chemical modifications, and low nucleic acid yield. iRepertoire’s optimized workflow—combining Covaris extraction, proprietary dam-PCR technology, and RepSeq+ seven-chain multiplex sequencing—overcomes these barriers to deliver reliable, quantitative immune repertoire profiling.

Download the case study to learn how to get the most out of precious FFPE samples for T cell and B cell immune monitoring analysis.