Technology
arm-PCR
AMPLICON RESCUED MULTIPLEX PCR
Ultra-Sensitive Single-Chain TCR/BCR Amplification
iRepertoire’s proprietary Amplicon Rescued Multiplex PCR (arm-PCR) technology uses hundreds of VDJ-specific primers to amplify any individual B or T cell receptor chain repertoire in a single sample, for cost-efficient insights into the immune repertoire
Why arm-PCR?
- Ultra-sensitive detection of rare TCR/BCR clonotypes
- Optimized for low-input samples, including single cells
- Hundreds of V(D)J-specific primers for broad chain coverage
- Cost-efficient profiling of any individual receptor chain
- Proven chemistry adapted from pathogen detection technologies
Unmatched utility
- Full BCR/TCR identity and chain usage
- CAR-T development
- Hybridoma sequencing
- Antibody identification
- Lymphocyte tracking
- Analyze rare cell populations or cell line
- sequencing
- And more!
Want to learn more?
Reach out for a consultation and let us help you customize your experiments with arm-PCR.
Simple, yet sensitive
Arm-PCR occurs in just two stages:
Highly sensitive RT-PCR:
Nested inside and outside primers selectively amplify all V- and C- genes and incorporate communal adaptors.
Highly specific PCR:
Following rescue, only target amplicons, which contain 5’ and 3’ communal adaptors, are exponentially amplified.
Whether you are looking to analyze immune repertoires or gain insight at the single cell level, iRepertoire has the service and expertise to help!
Select Repseq for immune repertoire analysis. This service allows for semi-quantitatively and inclusively amplifying all of the expressed V(D)Js in B or T cells in a single sample.
Select iPair for single cell resolution. This service allows for capturing physically paired variable regions in T and B cells.
See how arm-PCR technology was utilized in this case study
