Dimer Avoided Multiplex PCR
The adaptome, amplified in a single tube
Sequence all 7 B and T cell chains with dimer-avoided multiplex PCR (dam-PCR). This innovative technology uses a patent-pending approach to remove unwanted side product formation during PCR. Dam-PCR increases the signal of particular targets, allowing for unprecedented multiplexing power and all-inclusive sequencing of the immune adaptome.
Dam-PCR is at the core of our RepSeq+ services.
Dam-PCR excels at:
- Multichain analysis
- Clonotype frequency measurement
- Dimer removal
- Bias reduction
- Error detection
Highly specific amplification
The key to dam-PCR’s specificity is a stringent clean-up step that occurs after the first and second rounds of binding and extension. All unused, sequence-specific primers are removed during clean-up. The final enrichment phase of the PCR uses primers targeting communal tags that are added in the first two cycles.
Dam-PCR is compatible with the addition of unique molecular identifiers (UMI), which tag individual mRNA molecules. UMIs help identify PCR and sequencing errors for a more quantitative picture of the immune repertoire. Even without UMIs, dam-PCR is highly quantitative because the reaction is focused on the amplicon of interest.
Reduce costs, reduce time
In addition to services, dam-PCR for immune repertoire analysis is available through the automated iR-Flex System. You can expect to reduce assay time and labor with a 15 minute set-up and a 5 minute library retrieval protocol.
Our technology is also the most cost-effective and cost-saving technology on the market because of the multi-chain approach. Save time and money when incorporate dam-PCR into your workflow.
Dam-PCR has applications beyond the immune repertoire and can be customized to be catered to your needs. Current uses include:
Flexible sample input and compatibility with both gDNA and RNA, dam-PCR can be used for 4-digit HLA typing on gDNA and detecting cancer fusions on both gDNA and RNA under the same assay conditions.
Cell line contamination assessment assays:
With the inclusion of unique molecular identifiers on gDNA, cell counts can be estimated and also used to determine the concentration of potential cell line contaminants.
Because dam-PCR provides a holistic view of the immune repertoire, dam-PCR can be used in a wide range of clinical and research applications. Amplify your studies in any of the following areas with dam-PCR:
- Somatic hypermutation status
- Clonotype tracking
- Translational and basic research
- Infectious disease
- Autoimmune disorders
- And more
Check out our eBook for more information on dam-PCR and our tools for immune sequencing: