Technology

Inspiring clinically-actionable discoveries in immune biology

Quantitative TCR and BCR Sequencing for Bulk Repertoires and Single-Cell Paired-Chain Analysis

An immune repertoire is the sum of functionally diverse T and B cells in a body at any given moment. Sampling the immune repertoire provides both a snapshot and a historical record of immune function, enabling an advanced understanding of disease and treatment response.

Over the last 10 years, iRepertoire has developed the gold standard technologies for immune repertoire sequencing and continues to innovate new solutions for profiling the adaptome. Our core technologies provide the ability to amplify all of the expressed V(D)Js in B or T cells, and we offer the only technology that can measure all 7 immune chains in a single reaction. iRepertoire is committed to the creation of cutting-edge technology that adapts to the ever-changing immune sequencing landscape.

arm-PCR

Amplicon Rescue Multiplex PCR

arm-PCR inclusively amplifies all of the expressed V(D)Js in B or T cells for cost-efficient insights into the expressed immune repertoire. arm-PCR is ideal for capturing rare cell types, tetramer analysis, and single cell applications even for low volume and difficult to handle sample types.

dam-PCR

Dimer avoided multiplex PCR

dam-PCR enables highly quantitative amplification of any combination of TCR and BCR chains (alpha, beta, delta, gamma, IgH, and kappa/lambda) in a single reaction. dam-PCR is ideal for isotype and clonotype frequency analysis, hyper-mutation status, and FFPE-damaged samples.

Streamlined single cell immune sequencing for paired chain and phenotypic analysis

V(D)J RNA amplified and sequenced

Our iPair single cell immune sequencing technology is the first commercially available service specifically designed for capturing physically paired variable regions in T cells and B cells. Our iPair+ single cell immune phenotyping technology allows for co-expression analysis of over 100 phenotypic genes.

iPair Applications

Paired chain analysis
CAR-T development
Lymphocyte tracking
Antibody reverse engineering
Rare cell analysis

Enabling flexible inputs, more insights, and efficiency

iRepertoire immune sequencing | Competitor analysis

COVERAGE

	iRepertoire	Competitor A	Competitor B
Technology	arm-PCR and dam-PCR	multiplex-PCR	template switch
Bulk sequencing available?	Yes	Yes	Yes (10K cell max)
Single Cell Sequencing Available?	Yes	No	Yes
human CDR	CDR1, CDR2, & CDR3	CDR3 only	CDR1, CDR2, & CDR3
mouse CDR	CDR2 & CDR3	CDR3 only	CDR1, CDR2, & CDR3
IGH	Human & Mouse	Human & Mouse	Human & Mouse
IGL	Human & Mouse	Not Covered	Human & Mouse
IGK	Human & Mouse	Not Covered	Human & Mouse
TCRB	Human & Mouse	Human & Mouse	Human & Mouse
TCRA	Human & Mouse	Human	Human & Mouse
TCRD	Human & Mouse	Human	Human & Mouse
TCRG	Human & Mouse	Human	Human & Mouse
BCR isotype	Yes	No	Yes
UMI available?	Yes	No	Yes
Input (RNA/DNA)	RNA or DNA	DNA	RNA
Price	$	$$	$$$

Coverage
iRepertoire
Technology	arm-PCR and dam-PCR
BULK SEQUENCING AVAILABLE?	Yes
SINGLE CELL SEQUENCING AVAILABLE?	Yes
HUMAN CDR	CDR1, CDR2, & CDR3
MOUSE CDR	CDR1, CDR2
IGH	Human & Mouse
IGL	Human & Mouse
IGK	Human & Mouse
TCRB	Human & Mouse
TCRA	Human & Mouse
TCRD	Human & Mouse
TCRG	Human & Mouse
BCR ISOTYPE	Yes
UMI AVAILABLE?	Yes
INPUT (RNA/DNA)	RNA or DNA
PRICE	$
Competitor A
Technology	multiplex-PCR
BULK SEQUENCING AVAILABLE?	Yes
SINGLE CELL SEQUENCING AVAILABLE?	No
HUMAN CDR	CDR3 only
MOUSE CDR	CDR3 only
IGH	Human & Mouse
IGL	Not Covered
IGK	Not Covered
TCRB	Human & Mouse
TCRA	Human
TCRD	Human
TCRG	Human
BCR ISOTYPE	No
UMI AVAILABLE?	No
INPUT (RNA/DNA)	DNA
PRICE	$$
Competitor B
Technology	template switch
BULK SEQUENCING AVAILABLE?	Yes (10K cell max)
SINGLE CELL SEQUENCING AVAILABLE?	Yes
HUMAN CDR	CDR1, CDR2, & CDR3
MOUSE CDR	CDR1, CDR2, & CDR3
IGH	Human & Mouse
IGL	Human & Mouse
IGK	Human & Mouse
TCRB	Human & Mouse
TCRA	Human & Mouse
TCRD	Human & Mouse
TCRG	Human & Mouse
BCR ISOTYPE	Yes
UMI AVAILABLE?	Yes
INPUT (RNA/DNA)	RNA
PRICE	$$$