Technology
arm-PCR
AMPLICON RESCUED MULTIPLEX PCR
The adaptome, amplified in a single tube
Sequence an immune repertoire with amplicon rescued multiplex PCR (arm-PCR). iRepertoire’s
proprietary arm-PCR technology uses hundreds of VDJ-specific primers to amplify all of the expressed B and T cell receptor genes in a single sample for cost-efficient insights into the immune repertoire.
arm-PCR excels at:
- Inclusive immune sequencing: Hundreds of thousands of CDR3s amplified in a single experiment
- Capture rare cell types: Identify rare clonotypes in large cell populations
- Flexible: Compatible with low cell counts, low sample volumes and difficult sample types
Unmatched utility
- Full BCR/TCR identity and chain usage
- CAR-T development
- Hybridoma sequencing
- Antibody identification
- Lymphocyte tracking
- Analyze rare cell populations or cell line
- sequencing
- And more!
Want to learn more?
Reach out for a consultation and let us help you customize your experiments with arm-PCR.
Simple, yet sensitive
Arm-PCR occurs in just two stages:
Highly sensitive RT-PCR:
Nested inside and outside primers selectively amplify all V- and C- genes and incorporate communal adaptors.
Highly specific PCR:
Following rescue, only target amplicons, which contain 5’ and 3’ communal adaptors, are exponentially amplified.
Whether you are looking to analyze immune repertoires or gain insight at the single cell level, iRepertoire has the service and expertise to help!
Select Repseq for immune repertoire analysis. This service allows for semi-quantitatively and inclusively amplifying all of the expressed V(D)Js in B or T cells in a single sample.
Select iPair for single cell resolution. This service allows for capturing physically paired variable regions in T and B cells.
See how arm-PCR technology was utilized in this case study
