Amplicon rescued multiplex PCR
An entire immune repertoire amplified in a single tube
iRepertoire’s proprietary arm-PCR technology uses hundreds of VDJ-specific primers to amplify all of the expressed B and T cell receptor genes in a single sample for cost-efficient insights into the immune repertoire.
arm-PCR occurs in two stages:
- Highly sensitive RT-PCR: Nested inside and outside primers selectively amplify all V- and C- genes and incorporate communal adaptors.
- Highly specific PCR: Following clean-up, only target amplicons, which contain 5' and 3' communal adaptors, are exponentially amplified.