Technology

arm-PCR

iRepertoire’s proprietary Amplicon Rescued Multiplex PCR (arm-PCR) technology uses hundreds of VDJ-specific primers to amplify any individual B or T cell receptor chain repertoire in a single sample, for cost-efficient insights into the immune repertoire

Why arm-PCR?

Ultra-sensitive detection of rare TCR/BCR clonotypes
Optimized for low-input samples, including single cells
Hundreds of V(D)J-specific primers for broad chain coverage
Cost-efficient profiling of any individual receptor chain
Proven chemistry adapted from pathogen detection technologies

Unmatched utility

Full BCR/TCR identity and chain usage
CAR-T development
Hybridoma sequencing
Antibody identification
Lymphocyte tracking
Analyze rare cell populations or cell line
sequencing
And more!

Want to learn more?

Reach out for a consultation and let us help you customize your experiments with arm-PCR.

Simple, yet sensitive

Arm-PCR occurs in just two stages:

Highly sensitive RT-PCR:
Nested inside and outside primers selectively amplify all V- and C- genes and incorporate communal adaptors.

Highly specific PCR:
Following rescue, only target amplicons, which contain 5’ and 3’ communal adaptors, are exponentially amplified.

Arm-PCR is the core technology of our  RepSeq+  and iPair service.

Whether you are looking to analyze immune repertoires or gain insight at the single cell level, iRepertoire has the service and expertise to help!

Select Repseq+ for immune repertoire analysis. This service allows for semi-quantitatively and inclusively amplifying all of the expressed V(D)Js in B or T cells in a single sample.

Select iPair for single cell resolution. This service allows for capturing physically paired variable regions in T and B cells.

arm-PCR

AMPLICON RESCUED MULTIPLEX PCR Ultra-Sensitive Single-Chain TCR/BCR Amplification

Unmatched utility

Want to learn more?

Simple, yet sensitive

Arm-PCR is the core technology of our  RepSeq+  and iPair service.

See how arm-PCR technology was utilized in this case study

Read our brochure to more about arm-PCR and single cell sequencing: Single cell brochure

arm-PCR

AMPLICON RESCUED MULTIPLEX PCR Ultra-Sensitive Single-Chain TCR/BCR Amplification

Unmatched utility

Want to learn more?

Simple, yet sensitive

Arm-PCR is the core technology of our RepSeq+ and iPair service.

See how arm-PCR technology was utilized in this case study

Read our brochure to more about arm-PCR and single cell sequencing: Single cell brochure

Arm-PCR is the core technology of our  RepSeq+  and iPair service.