Dimer Avoided Multiplex PCR

The adaptome, amplified in a single tube

Sequence all 7 B and T cell chains with dimer-avoided multiplex PCR (dam-PCR). This innovative technology uses a patent-pending approach to remove unwanted side product formation during PCR. Dam-PCR increases the signal of particular targets, allowing for unprecedented multiplexing power and all-inclusive sequencing of the immune adaptome.

Dam-PCR is at the core of our RepSeq+ services.

Dam-PCR excels at:

Multichain analysis
Clonotype frequency measurement
Dimer removal
Bias reduction
Error detection

Highly specific amplification

The key to dam-PCR’s specificity is a stringent clean-up step that occurs after the first and second rounds of binding and extension. All unused, sequence-specific primers are removed during clean-up. The final enrichment phase of the PCR uses primers targeting communal tags that are added in the first two cycles.

Unbiased amplification

Dam-PCR is compatible with the addition of unique molecular identifiers (UMI), which tag individual mRNA molecules. UMIs help identify PCR and sequencing errors for a more quantitative picture of the immune repertoire. Even without UMIs, dam-PCR is highly quantitative because the reaction is focused on the amplicon of interest.

Reduce costs, reduce time

Our dam-PCR technology is the most cost-effective and cost-saving technology on the market because of the multi-chain approach. Save time and money with incomparable results when you incorporate dam-PCR into your workflow.

Applications

Because dam-PCR provides a holistic view of the immune repertoire, dam-PCR can be used in a wide range of clinical and research applications. Amplify your studies in any of the following areas with dam-PCR:

Somatic hypermutation status
Clonotype tracking
Translational and basic research
Oncology
Infectious disease
Autoimmune disorders
And more

Check out our eBook for more information on dam-PCR and our tools for immune sequencing: