High-precision analysis for confident results and deep insights
iRepertoire’s RepSeq+ service utilizes our dam-PCR technology, which allows for any combination of TCR and BCR chains (TCR-alpha, TCR-beta, TCR-delta, TCR-gamma, BCR-IgHeavy, and BCR-kappa/lambda) to be amplified within a single reaction.
Multichain analysis in one single reaction mix is beneficial in that it saves rare and/or precious samples by using less RNA than traditional methods. Dam-PCR also allows for the detection of unique mRNA transcripts, which provides reduced bias, better error removal, and more precise data on clonotype frequency.
One chain versus multi-chain
With RepSeq+, the sequencing reads devoted to a particular chain are determined by the natural state of the sample, which allows for calculations of BCR to TCR expression and clonotype ratios; this should be taken into consideration prior to chain selection for examination in the assay.
For instance, if you want information for delta/gamma TCRs from whole blood, using a 7-chain assay would not be advised since the delta/gamma frequency is naturally lower in whole blood (~5%) for most individuals. For this case, we would either recommend a 1- or 2-chain RepSeq+ analysis, or the use of our more inclusive, single chain RepSeq service, which relies on iRepertoire™’s arm-PCR technology.
RNA sample submission requirements
RepSeq+ | Dam-PCR
| Minimum concentration | Minimum mass | Optimum concentration | Optimum mass | Minimum volume | |
|---|---|---|---|---|---|
| FFPE | 5 ng/uL | 75 ng | 20 ng/uL | 300 ng | 15 uL |
| Solid Tissue | 5 ng/uL | 75 ng | 50 ng/uL | 1000 ng | 15 uL |
| PBMC / Whole Blood | 5 ng/uL | 75 ng | 50 ng/uL | 1000 ng | 15 uL |
| Sorted Cell Populations | 1 ng/uL | 15 ng | 10 ng/uL | 200 ng | 15 uL |
How to order
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