iPair+: Coupling single cell phenotypic data with repertoire analysis
iPair+ customizable human and mouse panels include markers for over 100 popular phenotypic genes
iPair+ helps you make connections between repertoire genotype and phenotypes.
With iPair+, you can choose from a large list of popular targets or customize your own. Our data analysis tools will help you interpret and present your phenotypic data through visualization tools such as T-SNE plots and heat plots.
Gene targets include markers for:
- T-cell related genes
- Macrophages related genes
- Dendridic cell related genes
- Netrohil related genes
- Eosoinophil related genes
- Transcription factors
- Surface markers
2 ways to use iPair+
- Coamplify using gene-specific primers in the same well as the VDJ receptor (human only)
- Amplify with oligo-dT primers followed by phenotypic sizing primers in a separate plate
Compare: Gene-specific Panel vs. Oligo DT panel
|Gene-specific panel||Oligo DT|
|Number of markers||Up to 30||100+|
|Customization||select any of the 30 available markers (validation required)||additional markers can be added (validation required)*|
|Advantage||Lower cost||Control read depth|
|Data analysis||T-SNE plots available||T-SNE plots, frequency of RNA|
*Please request the target lists. There is one for gene-specific panelandone foroligo-dT.
The main advantage of using gene specific primers is affordability. The oligo-dT approach requires a separate plate, but it allows for more user control of the sequencing depth of phenotypic targets. The single plate approach can result in drop out of the VDJ pair because highly expressed targets can occupy too many reads. We recommend using the Oligo-dT approach only if you are going to include a relatively small number of phenotypic primers.
A personalized phenotyping primer mix can also be made. Fee applies to every 20 plates or until the mix expires (whichever comes first). Oligo dT amplification fees apply. Additional sequencing recommended.