Are you looking for a customized NGS assay?
iRepertoire simplifies immune sequencing
Our experienced immune sequencing scientists will work with you to develop a service plan suited to your project goals, including handling of rare and difficult to work with sample types and custom data outputs.
What our customers say
“The best features of iRepertoire’s service are the custom repertoire analytics and applications for multiple research projects and models.”
Pranav Murthy, University of Pittsburgh Medical Center
“I would recommend iRepertoire’s services based on the ability to modify and customize the sequencing process to a researcher’s needs.”
Robert Maul, National Institutes of Aging
PHAGE DISPLAY AND SCFV LIBRARY SEQUENCING
Swiftly screen the diversity of phage displays or scFv libraries
Utilizing our extensive experience with NGS and immune repertoire analysis, we can quickly assess the diversity of phage and scFv libraries. We custom design NGS-compatible primers to capture from the linker or plasmid region to the beginning of the constant gene to create a tailored NGS assay for routine monitoring of your expression experiments. Using only the plasmid library or extracted RNA from the expressing cells, we return mapped, high-quality Illumina NGS data through our web-based data analysis platform, iRweb.
NGS LIBRARY AUTOMATION
Transform bench top NGS into fully automated assays
iRepertoire™ offers a custom service to convert bench top NGS assays onto the automated iR-Flex System. All of the reaction chemistry and processing steps – including amplicon clean-up – are contained within the cassette, which helps prevent human error, reduces the risk of sample contamination, and results in fewer labor- and time-intensive tasks. These assays are either based on amplicon-rescued-multiplex PCR (arm-PCR) technology or dimer-avoided-multiplex-PCR technology (dam-PCR) depending on assay requirements, determined on a case-by-case basis.
CUSTOM NGS ASSAY DEVELOPMENT
Harness the power of NGS and accelerate experimental results for any application
iRepertoire can work with any original idea to help plan the project all the way through NGS data generation. Our group is composed of expert immunologists and scientists who can assist you in converting an existing bench top assay into an NGS compatible assay. Assays can also be automated on the iR-Flex system.
Recent custom projects include:
- “mini-bulk” pooled single cell sequencing to track virus-reactive T cells
- Next-generation sequencing of hybridomas
- Hybridoma sequencing – digitize existing hybridoma assets
- Custom cancer mutation panels
- HLA typing
- Cell therapy process monitoring tools
- Custom phage display monitoring