Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™
Quantitative gDNA TCR/BCR Sequencing with RepSeq IQ™
Services | Immune Repertoire Analysis
RepSeq utilizes our patented arm-PCR technology which uses V-primer cycling to inclusively amplify all of the expressed V(D)Js in B and T cells in one reaction with unprecedented sensitivity. We recommend using RepSeq if your samples have low cell counts (<50,000 cells).
Discover rare clonotypes with low cell countsWe currently offer RepSeq services for all human and mouse TCR and BCR chains.
RepSeq+ utilizes our patent-pending dam-PCR technology which uses iterative rounds of amplification and amplicon recovery to selectively amplify any or all of the 7 TCR and BCR chains. The use of unique molecular identifiers enables detection of individual mRNA transcripts for improved error removal and more precise data on clonotype frequency.
Confident results for applications requiring high error detection| RepSeq Arm-PCR | RepSeq+ Dam-PCR | |
|---|---|---|
| Species covered | Human and mouse | Human and mouse (TCR only) |
| Chains covered | TCR-alpha, TCR-beta, TCR-delta, TCR-gamma | TCR-alpha, TCR-beta, TCR-delta, TCR-gamma |
| BCR-Ig Heavy, BCR-kappa/lambda | BCR-Ig Heavy, BCR-kappa / lambda | |
| Rare clone discovery | +++++ | +++ |
| Primers available | Short read and long read | Long read only |
| In-house kits available? | Automated and manual | Automated |
| Compatibility in one reaction | 1 chain per reaction | 1–7 chains per reaction |
| Dual index | No | Yes |
| Service: repeat amplification? | One free repeat attempt if first process fails | Technical replicate included for each sample plus free repeat if first process fails |
| Free basic data analysis | Yes | Yes |
| UMI quantification on RNA expression level | No | Yes |
| Sequencing | Sold separately | |