Cost-effective solutions for discovering rare clonotypes
iRepertoire’s RepSeq service (formerly AMP2Seq) utilizes our patented arm-PCR technology, which uses hundreds of VDJ-specific primers in one reaction to semi-quantitatively and inclusively amplify all of the expressed V(D)Js in B or T cells from a single sample.
RepSeq is our most cost-efficient solution to gain insight into the expressed immune repertoire for your chain(s) of choice. Because only one chain is amplified per reaction, CDR3 discovery is not affected by the relative chain frequency. However, as long as enough starting material is supplied, multiple chains can be amplified from the same RNA sample.
RNA sample submission requirements
|Minimum concentration||Minimum mass||Optimum concentration||Optimum mass||Minimum volume|
|FFPE||5 ng/uL||75 ng||20 ng/uL||300 ng||15 uL|
|Solid Tissue||5 ng/uL||75 ng||50 ng/uL||1000 ng||15 uL|
|PBMC / Whole Blood||5 ng/uL||75 ng||50 ng/uL||1000 ng||15 uL|
|Sorted Cell Populations||1 ng/uL||15 ng||10 ng/uL||200 ng||15 uL|