MiSeq machines

Investigating the full clonal diversity of an immune repertoire requires a uniquely tailored sequencing approach. Most immune sequencing chemistries on the market rely on some variation of a technology called template switching, which starts with untargeted amplification of all of the RNA in a given sample. iRepertoire’s proprietary multiplex PCR technologies use primers that are…

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pcr strip and biological microscope

Roughly half the papers published so far for immune repertoire sequencing use genomic DNA samples, while the other half use RNA. Although we have primers that work for both sample types, our preferred originating template is RNA which we use to create an immune specific cDNA template. The benefits using of RNA for immune sequencing…

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MiSeq machines

When planning your repertoire sequencing experiment, you’ll need to consider the appropriate read depth for your samples. Sequencing depth/coverage is impacted by the length of the reads, the sequencing platform, and whether you’re using single end or paired end sequencing. More coverage is always better, but costs tend to increase with sequencing coverage. Additionally, pooling…

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DNA samples are loaded to 96-well plate for PCR analysis

Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.

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iRepertoire poster entitled iR-HLA cassette: An automated and integrated solution for NGS based HLA-typing

Dig into our knowledge base through exploring our immune research posters. Here you can view and download our recently presented posters.

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graphic of cancer cell, shown in orange, and lymphocytes, shown around the cancer cell in blue

Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.

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The makeup of "whole blood" infographic. Whole blood contains red blood cells (RBCs), white blood cells (WBCs), platelets, and plasma. Plasma contains protein and additional nutrients. The WBCs include both peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells (PMNCs). Lymphocytes (T cells, B cells, NK cells), monocytes, and macrophages make up the PBMCs, while neutrophils, eosinophils, basophils, and mast cells make up PMNCs.

There are various sample collection methods researchers can utilize for immune repertoire studies. This document’s purpose is to define, compare, and contrast several methods of sample collection and downstream storage options that we currently use in-house as it applies to whole blood. This document is not a comprehensive review of all possible collection methods but…

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Biotech sample brown cardboard shipping package

iRepertoire can help with your immune repertoire sequencing project for any and all steps from nucleic acid extraction, library preparation, sequencing (of a library made with iRepertoire’s Reagent Systems), and finally data analysis. Regardless of where you find yourself in the workflow, this page describes the best practices for shipping samples for service, receiving products…

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Samples can be transferred to iRepertoire for library preparation and sequencing at any stage in the immune repertoire sequencing workflow from nucleic acid extraction to amplification to sequencing. Additionally, material can be sourced from a variety of different sample types. This page provides guidelines for sample submission whether you are planning to rely on iRepertoire…

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Experienced businesswoman reading publication via laptop computer making research for planning startup at desktop,female secretary checking mail on netbook organizing working process in office

Dig into our knowledge base in this linked reference list that brings all our publications and patents together in one comprehensive resource.

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