Posts by sambavince
iRepertoire’s iR-Complete Dual Index Primer Kits for RNA and gDNA
iRepertoire’s iR-Complete Dual Index Kits will replace both the iR-Profile and Bulk Primer kits to simplify reagent ordering and promote scalability of your immune sequencing experiments. Dual index adaptors for improved scalability The previous iR-Profile kit contained 10 uniquely-barcoded reactions with primers tailored to the species and chains of the user’s choice, along with reagents…
Read MorePooling samples
Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.
Read MorePosters
Dig into our knowledge base through exploring our immune research posters. Here you can view and download our recently presented posters.
Read MoreCell-sorting Guide
Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.
Read MoreHow to perform immune repertoire sequencing
Capturing the full clonal diversity of the immune repertoire can reveal important information about the pathology and prognosis of disease. Learn how now.
Read MoreiRepertoire’s immune sequencing services
Immune repertoire sequencing: RepSeq and RepSeq+ iRepertoire’s sequencing services RepSeq and RepSeq+ complement our amplification technologies arm-PCR and dam-PCR, respectively. Because arm-PCR and dam-PCR have different strengths, the decision of whether to use RepSeq or RepSeq+ will depend on the nature of your specific project. Arm-PCR uses internal and external nested primers for extremely inclusive…
Read MoreNGS considerations: coverage, read length, multiplexing
Plan your NGS experiment to adjust cost and amount of sequencing data to best answer your experimental questions. Dive into the top NGS considerations here.
Read MoreiPair: iRepertoire’s single cell immune sequencing service
Why single cells? Information related to the physical, cognate pairing of TCR-alpha and -beta chains or Ig-heavy and -light chains is lost once RNA extraction is performed on a bulk cell sample. iPair, our single cell immune sequencing service, was developed so that the repertoire of individual cells could be profiled through capture of chain…
Read MoreiRepertoire’s data analysis services
All of our services come with complimentary basic data analysis through our proprietary pipeline, which then outputs data to iRweb. iRweb is a web-based RepSeq bioinformatic software that allows researchers to have all of the following services and analyses performed on their samples: Barcode demultiplexing The number of CDR3s captured in the library and the…
Read MoreiRweb: Technical notes
Overview This page describes the technical steps involved in iRweb’s bioinformatic pipeline. If you are unfamiliar with data analysis for next-generation sequencing, we suggest you visit our NGS overview page. For a general introduction to iRweb, see our Learning Center article about data analysis. For more details about iRweb than what can be found on…
Read More