Choice of starting template: RNA vs gDNA vs cDNA

pcr strip and biological microscope

Roughly half the papers published so far for immune repertoire sequencing use genomic DNA samples, while the other half use RNA. Although we have primers that work for both sample types, our preferred originating template is RNA which we use to create an immune specific cDNA template. The benefits using of RNA for immune sequencing…

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Choosing your read depth

MiSeq machines

When planning your repertoire sequencing experiment, you’ll need to consider the appropriate read depth for your samples. Sequencing depth/coverage is impacted by the length of the reads, the sequencing platform, and whether you’re using single end or paired end sequencing. More coverage is always better, but costs tend to increase with sequencing coverage. Additionally, pooling…

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Pooling samples

DNA samples are loaded to 96-well plate for PCR analysis

Prepare for single-cell amplification and sequencing. Identify how to perform these steps either in-house or as an iRepertoire service.

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Blood collection and sample considerations

There are various sample collection methods researchers can utilize for immune repertoire studies. This document’s purpose is to define, compare, and contrast several methods of sample collection and downstream storage options that we currently use in-house as it applies to whole blood. This document is not a comprehensive review of all possible collection methods but…

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Shipping samples, receiving products, and submitting data

Biotech sample brown cardboard shipping package

iRepertoire can help with your immune repertoire sequencing project for any and all steps from nucleic acid extraction, library preparation, sequencing (of a library made with iRepertoire’s Reagent Systems), and finally data analysis. Regardless of where you find yourself in the workflow, this page describes the best practices for shipping samples for service, receiving products…

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Sample submission guide – bulk repertoire

Samples can be transferred to iRepertoire for library preparation and sequencing at any stage in the immune repertoire sequencing workflow from nucleic acid extraction to amplification to sequencing. Additionally, material can be sourced from a variety of different sample types. This page provides guidelines for sample submission whether you are planning to rely on iRepertoire…

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